Figure 4.

In vitro gene silencing effect of PLGA NPs.

Notes: BMDCs (2×10⁶ cells) were unmanipulated (no treatment) or transfected with empty PLGA NPs (mock) or with 100 nM, 300 nM, or 500 nM PINs for 24 hours. Then, the DCs were stimulated with 5 µg/mL PCNs for 24 hours. The cells were assessed for IL-10 mRNA expression via (A) RT-PCR and (B) real-time PCR (*P<0.05 vs the Mock group).

Abbreviations: PLGA, poly(lactic-co-glycolic acid); NP, nanoparticle; BMDC, bone marrow-derived dendritic cell; IL, interleukin; mRNA, messenger RNA; RT-PCR, reverse transcriptase–polymerase chain reaction; siRNA, small interfering RNA; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.