Figure 1.

Lectin pulldown assays. (A) Cell membrane lipid raft fractions isolated from control or STZ-diabetic rat IM tip were pooled and incubated with 30 μl of indicated agarose-bound lectins at 4°C overnight. After washing, the lectin precipitated samples were analyzed by immunoblotting with anti-UT-A1 antibody. (B) Densitometry analysis of UT-A1 protein bands from three separate experiments (n = 3). Each lectin precipitated UT-A1 was normalized with UT-A1 from input proteins (means ± SD, ^*P < 0.05, ^**P < 0.01, NS, no significance).