Table 2.
Newly developed polymorphic microsatellite markers. NA, number of alleles; HE, expected heterozygozity; HO, observed heterozygozity; FIS, inbreeding coefficient.
| locus | motif | primer sequence (5′–3′) | label | NA | allele size range (bp) | HE | HO | FIS |
|---|---|---|---|---|---|---|---|---|
| PCR multiplex 1 | ||||||||
| En-05a | (tgg)12 | F : ACGCAGCAGTAGGTACAGGAA R : TCCAACCACAACCACTAGCA |
PET | 5 | 145–166 | 0.343 | 0.140 | 0.601 |
| En-11 | (ac)14 | F : CCAACTCGTAGGTGTAGAGGAT R : CCGTCTCTTGTGAGTGTTGTG |
NED | 7 | 158–170 | 0.510 | 0.488 | 0.053 |
| PCR multiplex 2 | ||||||||
| En-08 | (ac)13 | F : CTGAGCGGTTGCAGAGTACC R : TTCCCGGCCAAAGTACTAAC |
6′FAM | 3 | 157–169 | 0.399 | 0.349 | 0.137 |
| En-10 | (tg)14 | F : CGTCCAGAAGATTCCTACCG R : TCTCTACCTCGTGTCTGCCT |
NED | 7 | 103–135 | 0.697 | 0.744 | −0.056 |
| En-15 | (ca)15 | F : CGATGAGATTCCGTAGACACC R : AACCCTAGCACCTCACATGC |
NED | 6 | 282–294 | 0.559 | 0.558 | 0.013 |
| En-19 | (tg)17 | F : TACATTCAAATTAGTCTTGTGCCC R : TTGGTCGAGCCTATCTGGTC |
PET | 6 | 177–195 | 0.579 | 0.605 | −0.033 |
| En-21a | (tac)18 | F : ACCATAACAACTACTACCACTACCACT R : TCGTAACTAAGTGTTGAATGTGAATTT |
VIC | 3 | 107–113 | 0.268 | 0.163 | 0.402 |
aThese loci were not used for the analysis of colony genetic structure.