Figure 2.
Expression of KCa3.1 in Purkinje Cell Axons
(A) Immunolabeling of KCa3.1 in cerebellar vermis (overview, left), PC soma, and dendrites (middle) and the center of the granule cell layer, which typically corresponds to the anatomical location where Purkinje cell axons have their first axonal branchpoint (70–100 μm) (right, arrowhead: potential axonal branchpoint).
(B) Co-immunolabeling of calbindin and KCa3.1 at PC somata and dendrites. Left, calbindin; Middle, KCa3.1; Right, merge. Maximum intensity projection.
(C) KCa3.1 staining along calbindin-positive axons in the granule cell layer. Maximum intensity projection.
(D) Calbindin-positive axonal branchpoint in the granule cell layer. Single optical plane, image deconvolved.