Figure 1.

Characterization of Blood Monocytes in P-407-Treated Mice

(A) Quantitative analysis of total monocyte numbers in B6 mice treated with P-407 or PBS for 28 days. Circulating monocytes were identified by their low SSC and co-expression of CD115 and CD11b.

(B) Representative dot plots showing the frequencies of Gr1^high and Gr1^low monocyte subsets at the end of the time course.

(C and D) Numbers (C) and relative frequencies (D) of Gr1^low and Gr1^high blood monocytes. P-407-treated mice kept on a chow diet had significantly fewer Gr1^low and more Gr1^high monocytes at each time point. Plots represent data pooled from four different experiments and show the mean values ± SE for at least seven mice per time point. The p value of the comparison between day 0 and day 28 is indicated (^∗p < 0.05; ^∗∗p < 0.01, and ^∗∗∗p < 0.001; unpaired t test).

(E) Frequency of Annexin V⁺/PI⁺ monocytes in the two monocyte subpopulations 7 and 14 days after P-407 treatment. Results are expressed as mean ± SE, n = 4 per time point (unpaired t test).

(F) Representative images of neutral lipid content in blood leukocytes after 28 days of PBS or P-407 treatment. Leukocytes were stained for neutral lipid accumulation using the LipidTox kit according to manufacturer’s instructions. Neutral lipid droplets are shown in green. Scale bar, 10 μm.

(G) Quantification of the lipid content. Data are expressed as mean fluorescent intensity (MFI) per cell (mean of three fields per mouse, 15 cells/field, and four mice per group; unpaired t test). Horizontal bars represent means. ns, nonsignificant.

See also Figures S1 and S2.