Figure 3.

In Vivo Behavior of CX3CR1^high Monocytes under P-407-Induced Dyslipidemia

Cx3cr1^gfp/+ mice were treated with PBS or P-407 for 7 days and ear dermis vasculature imaged by intravital microscopy.

(A) Examples of intravascular crawling cells (blue arrow) or tissue cells (white arrow) with PBS or P-407 treatment. Scale bars, 50 μm.

(B) Quantitative representation of the number of intravascular crawling cells per hour, crawling velocity and track displacement after each treatment. n = 3 per group. ^∗∗∗p < 0.001 from PBS control. ns, nonsignificant. Scale bars, 50 μm.

(C) Same as (A) and (B) except that representative mesentery vasculature from Cx3cr1^gfp/gfp or Cx3cr1^gfp/+ mice were imaged over 60 min. Region of interest (ROI) was selected which demonstrates gallery over time of CX3CR1^high cell accumulation at the endothelial interface (dotted line) following P-407 treatment. Representative of four mice; ^∗∗p < 0.01. Scale bar, 50 μm.

(D and E) As in (C), where (D) shows examples of tissue from an ROI (500 μm²) away from the venule showing accumulation of tissue GFP⁺ cells after P-407 treatment. Two mice representative of four. Scale bar, 100 μm. (E) Quantitative representation of the number of tissue GFP⁺ cells in PBS- and P-407-treated Cx3cr1^gfp/gfp or Cx3cr1^gfp/+ mice. n = 3 per group. ^∗p < 0.05 and ^∗∗p < 0.01.

See also Figure S4.