Figure 4.

Tissue Accumulation of CD68⁺ Cells under P-407 Treatment

(A) Representative photomicrographs of CD68 staining (brown) of PLP fixed liver, heart, and kidney sections after 7, 14, and 28 days of P-407 administration and after PBS treatment for 28 days. Magnification as indicated.

(B) Quantitative analysis of CD68⁺ cells in (A) specimens showing accumulation of CD68⁺ macrophages in the P-407-treated animals. For liver and heart, data are expressed as mean percentage ± SE of the brown-stained area in a selected field/total field area (five different fields per section). For the kidney, data represent mean percentage ± SE of the brown-stained glomerular area/total glomerular area for 20 glomeruli per section. Values represent the mean ± SE of at least four mice per group. p value by unpaired t test is shown (^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001).

(C) Representative images of the Ki-67 staining (green) and CD68 (red) of the sections (A) following 28 days of treatment with P-407 or PBS. Splenic sections were used as positive controls, and Ki-67⁺ cells are indicated with arrows. Scale bars, 75 μm.

See also Figure S4.