Figure 5.

Tracing Monocyte Subset Migration by Adoptive Transfer

(A) Gr1^lowGFP^high or Gr1^highGFP^low blood monocytes were sorted from CD45.2 Cx3cr1^gfp/gfp mice. 0.1 × 10⁶ Gr1^lowGFP^high or Gr1^highGFP^low sorted monocytes were injected intravenously into CD45.1.B6 mice. At 16 hr, mice were injected with CD11b antibody to exclude blood contamination. Mice were perfused with PBS, and organs (liver, heart, kidney, and spleen) were analyzed for monocyte migration. Dot plot overlays display total cells (gray) and CD45.2⁺CD11b⁻GFP⁺ cells (red).

(B) Quantitative representation of (A). Data are from two independent experiments (n = 4); values represent the mean ± SE of number of cells per gram of organ.

(C) B6 mice were treated with P-407 for 21 days or for 14 days followed by 7-day treatment with PBS. Mice treated with PBS for 21 days were used as controls. Data are expressed as percentage of Gr1^low and Gr1^high blood monocytes. Values represent mean ± SE, n = 4 mice per group. Significant p values are indicated (^∗∗∗p < 0.001; ns, nonsignificant; unpaired t test).

See also Figure S5.