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. 2015 Jul 15;11(9):1499–1519. doi: 10.1080/15548627.2015.1063764

Figure 6.

Figure 6.

Upregulation of proteins related to the UPR and autophagy. MCF-7 cells expressing RPLP0 shRNA, RPLP1 shRNA, RPLP2 shRNA, or NT shRNA vectors, untreated (DMSO) (A) or treated (B) with the autophagy inhibitor 3-MA (10 mM), were immunoblotted for the indicated proteins (phospho- or total). Expressions of p-EIF2AK3, p-EIF2S1, p-MTOR, p-AKT1, and p-MAPK1 were compared with those of total proteins (EIF2AK3, EIF2S1, MTOR, AKT1, and MAPK1, respectively). Expressions of ATF4, ATF6, and TP53 were compared with those of ACTB (used as a loading control). Parental MCF-7 cells treated with Sts (2 µM) were used as a control for apoptosis.