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. 2015 Jun 23;11(9):1652–1667. doi: 10.1080/15548627.2015.1059558

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Figure 5. — Effect of CDC42 on autophagy pathways. (A) Knockdown of CDC42 (or ATG5 as positive control) impairs formation of red-only structures in ptfLC3-transfected Huh7 cells (4 h starvation in EBSS). Colocalization of these structures with lysosomal marker LAMP1 (blue) only appears in cells transfected with negative control siRNA (si-neg). Treatment with lysosome inhibitor bafilomycin A₁ (Baf, 20 nM) served as positive control. Scale bar: 5 μm. (B) Knockdown of ATG5 and CDC42 was analyzed by western blotting. ACTB/β-actin served as loading control.