Figure 4 (See previous page).

Intermittent fasting (IF) worsens the autophagic impairment observed in lamp2 null mice. (A) Representative immunoblot depicting LC3 processing and SQSTM1 in cardiac extracts from young adult lamp2 null males (and littermate wild-type controls) treated with CQ or diluent to assess autophagic flux. (B and C) Quantification of LC3-II (B) and SQSTM1 (C) in mice treated as in A. N = 4 /group. * indicated P < 0.05 versus diluent treated wild-type control. P values are by one-way ANOVA. (D–F) Representative immunoblots (D) with quantitative analysis of LC3-II (E) and SQSTM1 (F) in lamp2 null mice subjected to intermittent fasting (for 5 wk) as compared with similarly treated littermate controls, and age-matched nonfasted lamp2 null and littermate wild-type mice. N = 4 to 6/group. P values depicted are by post-hoc test after one-way ANOVA. (G) Representative transmission electron microscopy images of cardiac tissues from lamp2 null mice and littermate wild-type mice subjected to intermittent fasting, or provided ad libitum access to food for 5 wk, on a fed day. N = 3 /group. Arrows indicate mitochondria with loss of cristal architecture, and arrowheads point to autophagic structures.