Figure 9.

Fasting and refeeding result in transcriptional regulation of autophagy-lysosome machinery genes. (A–F) Quantitative PCR analysis of relative abundance of representative autophagy-lysosome machinery gene transcripts, namely Map1lc3b (coding for LC3B; A), Sqstm1 (B), Becn1 (C), Lamp1 (D), Lamp2a (E) and Lamp2b (F) in adult male C57BL/6 mice subjected to 24 h of fasting, fasting for 24 h followed by refeeding for 24 h (fasted-refed), 48 h of fasting; and intermittent fasting for 3 and 6 wk (with samples procured on a fed day). Age-matched nonfasted controls were employed for each group. Control RNA for comparing the 24- and 48-h fasted RNA was prepared from the same nonfasted mouse hearts, and ran separately with each experimental group. N = 4 to 8/group. P value is by t test versus respective controls.