Figure 11.

Fasting induces nuclear translocation of TFEB in the myocardium. (A and B) Representative immunoblot (A) with quantification of nuclear TFEB (B, bands numbers from A) in nuclear and cytoplasmic subfractions from wild-type adult male C57BL/6 mouse hearts subjected to indicated durations of fasting and refeeding. Immunoblotting for GAPDH and Histone H3 was employed to detect enrichment of cytoplasmic and nuclear subfractions, respectively. N = 3 /group. P value is by post-hoc analysis after one-way ANOVA. (C) Quantitative PCR analysis of relative abundance of Tfeb transcript in mice subjected to 24 or 48 h of fasting (with age matched nonfasted controls); fasting for 24 h followed by refeeding for 24 h (fasted-refed). N = 4 to 8/group. P value is by t test vs. control. RNA from a common group of nonfasted mice was employed as control to compare transcript abundance in 24- and 48-h fasted samples.