Skip to main content
. 2015 Sep 14;11(8):1230–1246. doi: 10.1080/15548627.2015.1056968

Figure 2.

Figure 2.

Detachment from the ECM triggers growth arrest and apoptosis of intestinal epithelial cells. (A) IEC-18 cells were cultured attached to or detached form the ECM for 15 h and assayed for the distribution of the cells in phases of the cell cycle by flow cytometry. Percentage of the cells in the S phase of the cell cycle is shown. The numbers represent the average of 2 independent experiments plus the SD. (B) The cells were cultured as in (A), stained with propidium iodide (PI) and ANXA5 and assayed for ANXA5 and PtdIns binding by flow cytometry. Percent apoptosis was calculated as the sum of the percentages of ANXA5-positive-PI-negative cells (undergoing early stages of apoptosis) and ANXA5-positive-PtdIns-positive cells (undergoing late stages of apoptosis). The numbers represent the average of 2 independent experiments plus the SD. * Indicates that the p-value was less than 0.05.