Figure 8.

Lysine 243 of ATG3 is not required for the ability of ATG3 to kill attached intestinal epithelial cells. (A) IEC-18 cells were infected (+) or not infected (−) with 4 × 10⁴ viral particles per cell of the control retrovirus (cont virus) or ATG3-encoding retrovirus (ATG3 virus), or the virus encoding ATG3 in which K243 was mutated to arginine (ATG3KR virus), treated with 2 μg/ml puromycin for 24 h and assayed for ATG3 expression by western blot. CDK4 was used as a loading control. (B) IEC-18 cells treated as in (A) were assayed for survival as in Figure 5F. The numbers represent the average of triplicates plus the SD. This experiment was repeated twice with similar results. * indicates that the p-value was less than 0.05.