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. 2015 Sep 14;11(8):1293–1307. doi: 10.1080/15548627.2015.1058474

Figure 1.

Figure 1.

Intracellular survival and replication of B. pseudomallei in A549 cells. (A) Representative TEM image of control A549 cells. (B–D) A549 cells were infected with B. pseudomallei at an MOI of 10 for 2, 12, and 24 h, respectively. The scale bars are indicated. (B) Representative TEM images of intracellular autophagosomes within A549 cells at 2 h p.i. Arrows indicate autophagosomes. (C) TEM image of intracellular B. pseudomallei within A549 cells at 12h. (D) TEM images of intracellular B. pseudomallei within A549 cells at 24 h p.i. The left panel shows multinucleated giant cells. Boxed areas are shown as magnified images on the right panel. (E) Multiplication of B. pseudomallei in A549 cells. A549 cells infected with B. pseudomallei (MOI = 10:1) for 2, 4, 8, 16, 24, and 36 h were lysed, and intracellular bacteria were quantified after inoculation. The recovered viable B. pseudomallei were determined as CFU on a LB plate. Results shown are representative of 3 independent experiments.