Figure 7.

Downregulation of PEX1, SQSTM1, or NBR1 does not influence PEX5-EGFP-induced peroxisome removal. SV40T-MEFs were sequentially transfected with scrambled (NC1), PEX1-, SQSTM-, or NBR1-specific duplex siRNAs (DS) in combination or not with plasmids encoding mitochondria-targeted EGFP (marker for transfected cells) and (A, C) HsPEX5-EGFP or (B) HsPEX5 (for details, see Materials and Methods). One day later, the cells were fixed and processed for immunofluorescence microscopy with anti-PEX14 antibodies. Peroxisome degradation was quantified and plotted as in Figure 2B. The values above each bar represent the number of transfected cells analyzed per condition. A compilation of the results of at least 2 independent experiments (see Fig. S24) is shown. The “>50 peroxisomes” values from the (sub)panels were statistically compared with the value from the corresponding control (NC1) condition and found not to be statistically different.