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Baf does not affect localization of FLCN on lysosomes. C5.18 cells were differentiated into chondrocytes and treated with Baf for 48 h. Colocalization between lysosomes (visualized by LAMP2 antibody) and FLCN was explored by confocal microscopy (A). Quantification revealed no differences in lysosomal localization of FCLN upon Baf exposure (B). p = 0.7489, n = 3 independent experiments with approximately 400 cells analyzed in each experiment.
