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. 2015 Sep 30;4:11. doi: 10.1186/s13630-015-0020-2

Fig. 1.

Fig. 1

Flow diagram illustrating the cell culture conditions of HUVEC and HMEC-1 used in this study. First, during growth prior to confluence, cells are grown in either low or high serum media treatment. Upon confluence, cells were either fixed or subjected to serum starvation for 48 h or cobblestone treated (grown for an additional 5 days in the corresponding expansion media). All 12 combinations were assessed