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. 2015 Sep 30;4:11. doi: 10.1186/s13630-015-0020-2

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© Lim et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Single staining leads to false positive detection of primary cilia. Arl13b (green) and acetylated $α$ -tubulin (red) labelling in low (2 % FBS) serum HMEC-1, showing a lack of staining with arl13b but a false positive cilium labelled with 611b. Nuclei are labelled with Hoecsht (blue)