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. 2015 Sep 30;4:11. doi: 10.1186/s13630-015-0020-2

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© Lim et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Use of co-labelling to identify primary cilia HUVEC and HMEC-1 primary cilia identification using both axonemal marker arl13b (a, b) and acetylated $α$ -tubulin marker 611b (a′, b′, white arrows). (a″, b″) Co-localisation of both antibodies indicates presence of cilium. a-a′) HUVECs grown in high serum (20 % FBS) confluent. b, b″ HMEC-1s are grown in low serum (2 % FBS) then serum-starved (0 % FBS for 48 h)