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Factorial design of all formulations.
| Formulation | Factorial design | Calcium chloride (mL) | Chitosan (mg) |
|---|---|---|---|
| F1 | (−1, −1) | 1 | 2 |
| F2 | (−1, 0) | 1 | 3 |
| F3 | (−1, +1) | 1 | 4 |
| F4 | (0, −1) | 2 | 2 |
| F5 | (0, 0) | 2 | 3 |
| F6 | (0, +1) | 2 | 4 |
| F7 | (+1, −1) | 3 | 2 |
| F8 | (+1, −1) | 3 | 3 |
| F9 | (+1, +1) | 3 | 4 |
