Table 2.

Effect of preservatives on the transcorneal permeation of voriconazole from ophthalmic solutions through freshly excised goat cornea.

Preservatives	Amount of drug permeated in 120 min (mg)	% in vitro permeation	P app × 10−6 (cm/s)	Corneal hydration (%)
Control	0.047 ± 0.001	0.314 ± 0.003	1.73 ± 0.017	79.23 ± 0.30
Benzyl alcohol	0.046 ± 0.001	0.308 ± 0.002	1.70 ± 0.013	79.35 ± 0.24
PMA	0.052 ± 0.001†	0.349 ± 0.003†	1.93 ± 0.071†	78.33 ± 0.61
PMN	0.054 ± 0.001††	0.358 ± 0.003††	1.98 ± 0.071††	79.07 ± 1.76
Thiomersal	0.058 ± 0.001†††	0.384 ± 0.007†††	2.12 ± 0.021†††	79.96 ± 0.57
BAK	0.056 ± 0.002††	0.373 ± 0.012†††	2.06 ± 0.067†††	79.53 ± 0.03
EDTA	0.060 ± 0.001†††	0.400 ± 0.005†††	2.21 ± 0.025†††	79.37 ± 0.26
BAK + EDTA	0.317 ± 0.001††	2.120 ± 0.003††	30.72 ± 0.055†††	77.88 ± 0.15

Values are mean ± SE of 3 corneas in each group.

^†Statistically significant difference at P < 0.05; ^††statistically significant difference at P < 0.01; ^†††statistically significant difference at P < 0.001 from control (voriconazole 1.5%, pH 7.0 HP-β-CD based solution) as determined by one-way ANOVA followed by Dunnett's test.