Table 3.

Serial dilutions of the CVS-11 plasmid in real-time PCR (NDWD).

Dilution	Copy numbers/2 μL	CT-value 1	CT-value 2	CT-value 3	Mean ± SD	CV (%)
10−1	106	22.6	22.0	22.4	22.3 ± 0.31	1.37
10−2	105	25.1	25.2	25.3	25.2 ± 0.10	0.40
10−3	104	29.1	28.8	28.7	28.9 ± 0.21	0.72
10−4	103	32.9	32.9	32.8	32.9 ± 0.06	0.18
10−5	102	36.4	36.2	35.9	36.2 ± 0.25	0.70
10−6	101	47.5	38.6	39.0	41.7 ± 5.03	12.05
10−7	100	—	—	—	—	—

SD: standard deviation; CV: coefficient of variation.