Figure 1.

Inhibition of HEK293 cell proliferation by metformin (a) and rapamycin (b). 5000 cells per well were seeded in 96-well plates in MEM, cultured overnight and then exposed to increasing concentrations of metformin (Met) and rapamycin for 24 h. In combination with metformin treatments, 20 μM of compound C (Cc) was used. Cell proliferation was measured by the MTS cell proliferation assay. Composite results expressed as mean ± SEM of three independent experiments. (a) Mixed ANOVA: within subject effects of metformin concentration (F (5,85) = 52.48  P < 0.001), between subject effects of combination treatments with compound C F (1,17) = 52.15  P < 0.001. (b) ^* P < 0.05, compared with 100%.