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. 2014 Mar 25;2014:327640. doi: 10.1155/2014/327640

Figure 5.

Figure 5

(a) Rapamycin and metformin differentially affect high glucose-induced total protein synthesis. HEK293 cells were incubated with 5.5 mM D-glucose (Ctrl), 5.5 mM D-glucose + 24.5 mM mannitol (Man), and 30 mM D-glucose (HG) for 48 h. For the last 24 h, 100 nM rapamycin (Rap) and 8 mM metformin (Met) were added to HG-treated conditions. The results expressed as mean + SEM of four independent experiments. *** P < 0.001. (b) Metformin (Met) and rapamycin (Rap) differentially affect S6K phosphorylation in high glucose pretreated HEK293 cells. The cells were incubated with 5.5 mM (NG) or 30 mM D-glucose (HG) for 3 days. For the last 24 h, 8 mM metformin (Met) or 100 nM rapamycin (Rap) was added. The expression of the indicated proteins was analysed by western blotting. (c) Western blot results of the effect of metformin and high glucose on AMPK and S6K phosphorylation. HEK293 cells were cultured in normal medium supplemented with 5.5 mM D-glucose. At the indicated final concentrations, (mM) metformin (Met) and D-glucose (HG) were added for 24 h and three days, respectively.