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mTOR inhibition by rapamycin does not have a negative effect on p21 regulation. HEK293 cells were incubated with 100 nM rapamycin (Rap) for 24 h. Vehicle effects were controlled by 0.1% ethanol treatments. (a) Representative western blot image of p21 and β-actin expression. (b) Band intensity was quantified by densitometry. Data are arbitrary units (AU) expressed as means ± SEM (n = 6).
