Table 1.
Enzyme types used for human islet isolation.
| Enzyme types | Manufacture | Concentration | Digestion time (min) |
|---|---|---|---|
| Sigma type V collagenase | Sigma | 1 g/350 mL | 14 |
| BM Type P collagenase | Boehringer-Mannheim | 0.7 g/350 mL | 25 |
| Liberase HI | Roche Applied Science | 0.5 g/350 mL | 16 |
| Liberase MTF C/T, GMP grade | Roche Applied Science | 19 | |
| Collagenase | 0.5 g/350 mL | ||
| Thermolysin | 0.015 g/350 mL | ||
| Collagenase NB 1 (premium and GMP) | SERVA Electrophoresis GmbH | 14 | |
| Collagenase | 1600–2286 PZ units/350 mL | ||
| Neutral Protease | 200–286 DMC units/350 mL | ||
| VitaCyte C1 collagenase | VitaCyte | 20 | |
| CIzyme collagenase HA | 15–18 units/g tissue | ||
| CIzyme thermolysin | 1.25 DMC units/g tissue |
MTF: mammalian tissue free.
GMP: good manufacturing practice.
DMC: dimethylcasein.