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. 2015 Oct 1;11(10):e1005495. doi: 10.1371/journal.pgen.1005495

Fig 4. Analysis of the new fusion transcript in HsInv0379 breakpoint.

Fig 4

A. RNA-Seq reads mapped to an AC construct corresponding to BP1 (vertical red arrow) in an inverted chromosome reveal a fusion transcript present only in the four Std/Inv individuals (bottom) but not in the four Std/Std (top). Boxes highlight the new exon (yellow) and ZNF257 first exon (green). The structure of the fusion transcript reconstructed by Cufflinks [38] is shown below the RNA-Seq profiles. Small arrows indicate the approximate position of the primers used to validate the transcript. The coordinates of the new exon in the HG19 reference genome are also indicated. B. Analysis of fusion transcript expression by RT-PCR in several individuals. C. Quantification of the fusion transcript levels by qPCR in 15 Std/Std, 11 Std/Inv, and 1 Inv/Inv individuals.