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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Transplantation. 2015 Oct;99(10):2083–2094. doi: 10.1097/TP.0000000000000744

Figure 1. Immunological mechanisms behind the miHA vaccine.

Figure 1

Donor T cell activation to the miHA-encoded in the vaccine constructs occurs by several mechanisms (55). Direct transfection with the expression plasmids or transduction with rAd5 of donor dendritic cells and somatic cells occurs following vaccination. The constructs are shuttled to the nucleus (dotted lines). This is followed by transcription, translation, peptide processing, and expression of miHAs on MHC class I via the endogenous pathway to donor CD8 T cells directly from the dendritic cells and somatic cells (solid arrows). The transfected or transduced somatic cells act as an antigen reservoir, producing miHAs that undergo endocytosis by dendritic cells that are then presented by the exogenous pathway on MHC class II or through cross-presentation on MHC class I (solid arrows).