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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Kidney Int. 2015 May 20;88(4):754–763. doi: 10.1038/ki.2015.151

Figure 4. Trypanolytic activity for diverse APOL1 isoforms.

Figure 4

The results are expressed in percent of control growth in fetal calf serum for the following Trypanosoma brucei clones: T.b. rhodesiense SRA+ resistant clone (Tbr R) in black, T.b. rhodesiense SRA sensitive clone (Tbr S) in light gray, and T.b. gambiense (Tbg) in dark grey. Below each individual bar chart, the anti-APOL1 Western blot results confirm the presence of non-degraded APOL1 protein in the plasma sample. We tested the following APOL1 isoforms: G1 in homozygous state (bar chart 1) and heterozygous state with G2 (bar chart 2), G2 (bar chart 3), G3 (bar charts 4 and 5), rs2239785-A; p.150K (K, bar charts 6 and 7), and G0 or WT homozygous (bar chart 8). The only rs73885316-A; p.K264-containing sample was also homozygous for p.150K: the results are therefore similar to bar chart 6. Each bar chart is representative of several experiments. While none of the tested plasma killed T.b. gambiense, all of them killed T.b. rhodesiense devoid of SRA (Tbr S), our positive control. Only plasma from individuals carrying G1 or G2 variants could overcome the SRA-driven inhibition and kill T.b. rhodesiense R.