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. 2015 May 2;77(9):1109–1114. doi: 10.1292/jvms.14-0516

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©2015 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 3. — EYFP-cattle XLF accumulated quickly at DSBs induced by laser microirradiation. (A) The localization and accumulation of EYFP-cattle XLF at DSBs induced by 405 nm laser irradiation were examined. (B) Imaging of living EYFP-cattle XLF-transfected MDBK cells before (upper panel) and at 1 min after (lower panel) microirradiation. Left panel, EYFP-cattle XLF; right panel, differential interference contrast images (DIC). Arrowheads indicate the microirradiated sites. (C) Immunostaining of microirradiated EYFP-cattle XLF-transfected cells with anti-γH2AX antibody. The cells were fixed and stained with the anti- γH2AX antibody at 5 min postirradiation. Left panel, EYFP-cattle XLF; center panel, γH2AX image; right panel, merged image. (D) Time-dependent EYFP-cattle XLF accumulation in living cells (5-120 sec) after irradiation. Upper panel, EYFP-cattle XLF; lower panel, differential interference contrast images (DIC).