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. 2015 May 2;77(9):1137–1142. doi: 10.1292/jvms.14-0651

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©2015 The Japanese Society of Veterinary Science

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Fig. 3. — EYFP-mouse Ku70 accumulated with Ku80 immediately at DSBs induced by laser microirradiation. (A) Time-dependent EYFP-Ku70 accumulation in living cells (5–60 sec) after irradiation. (B, C) Immunostaining of microirradiated EYFP-mouse Ku70-transfected cells with the anti-γH2AX antibody (B) or anti-Ku80 antibody (C). At 5 min postirradiation, the cells were fixed and immunostained with each specific antibody. Left panels, EYFP-mouse Ku70 images (B, C); center panels, γH2AX (B) and Ku80 images (C); right panels, merged images (B, C). Arrowheads indicate the microirradiated sites.