Fig 4.

Analysis of transposable element activity in siSETDB1 injected embryos. A) Measurements of transcripts derived from the indicated clades of bovine transposable elements were measured in bovine blastocysts using q-RT-PCR. B) Measurements of transcripts derived from the indicated clades of bovine transposable elements measured in bovine fetal fibroblasts using q-RT-PCR. C–D) Measurements of bovine LINE1 transcripts in bovine 8-cell (C) and morula stage embryos (D) from Control, siNull and siSETDB1 treatment groups using q-RT-PCR. For A–D, samples were normalized to the geometric mean of GAPDH, YWHAZ & SDHA and graphed using the 2^{−ΔΔ CT} method [52]. Graphs are representative of four independent experiments. Error bars represent the SEM, * denotes p < 0.05.