Fig 5.

Analysis of global levels of H3K27 acetylation in siSETDB1 injected embryos. A) Representative confocal images of morula-stage embryos co-stained with antibodies recognizing H3K27ac (green) and the Hoechst DNA stain (blue). Images are at 20 × magnification. B) Average fluorescence intensity ratios were calculated for each embryo by dividing the staining intensity for H3K27ac by the intensity of DNA stain Hoechst. Ratios from each embryo were then averaged to obtain a mean ratio for each treatment group. Using these means, a one-way ANOVA model was constructed in order to determine differences between treatment groups. Bars displaying different letters were considered to be different (p < 0.05). Using a post-hoc Tukeys test, the differences between the siSetDB1 and Control and siNull groups were (p < 0.001). Error bars represent the SEM.