Figure 1. Loss of AID abrogates CpG methylation changes during GC transition.
(A) Combined CpG methylation of NB and GCB from WT mice (7 replicates; left) and Aicda-/- mice (6 replicates; right), determined by ERRBS using a 20% methylation difference threshold and FDR<0.001, Fisher's exact test. HypoDMCs are indicated in blue and hyperDMCs are indicated in yellow. (B) Number of DMCs between GCB and NB from WT and Aicda-/- mice determined by ERRBS using a 20% methylation difference threshold and FDR<0.001, Fisher's exact test. HypoDMCs are indicated in blue and hyperDMCs are indicated in yellow. A and B show that the DNA methylation changes after GC transition in WT mice are abrogated in Aicda-/- mice (C) Density plot showing delta methylation values (GCB % - NB %) determined by ERRBS in WT and Aicda-/- mice. Aicda-/- delta methylation values are decreased compared to WT, indicating less changes in methylation during GC transition. See also Figure S1.
