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. Author manuscript; available in PMC: 2016 Sep 29.
Published in final edited form as: Cell Rep. 2015 Sep 17;12(12):2009–2020. doi: 10.1016/j.celrep.2015.08.047

Figure 2. Diminished NMJ synaptic growth following lysosomal dysfunction is due to decreased activation of Rag/MTORC1.

Figure 2

(A) TRPML is required for vesicle fusion, and in the presence of lysosomal free amino acids, MTORC1 is activated resulting in the phosphorylation of downstream targets.

(B–G) Confocal images of larval NMJs from animals of the indicated genotypes stained with antibodies against HRP (green) and DLG (magenta). Scale bar shown in (B) also applies to (C–G).

(H–I) Bar graphs showing the average bouton numbers in animals of the indicated genotypes.

(J) Bar graph showing the percentage of the trpml1 adults carrying the indicated transgenes that eclose from the pupal cases.

“*”, “#”, and “¶” represent statistical significance. Abbreviations: n.s., not significant.