Figure 6. Drosophila and mouse models of Batten disease also show diminished neuronal JNK signaling.

(A–B) Confocal images of larval NMJs from animals of the indicated genotypes stained with antibodies against HRP (green) and DLG (magenta). Scale bar shown in (A) also applies to (B).

(C–D) Bar graphs showing the average bouton numbers in animals of the indicated genotypes.

(E) Western blots performed with larval brain extracts of the indicated genotypes probed with α-pJNK and α-tubulin primary antibodies.

(F) Bar graph showing the pJNK band intensities normalized to the tubulin band intensities in the indicated genotypes. The values shown are relative to the appropriate UAS controls.

(G and I) Coronal sections of E19.5 mouse brains of the indicated genotypes showing α-pJNK staining by immunohistochemistry. Scale bar shown in (G) also applies to (I).

(H and J) Higher magnification of the boxed regions from (G) and (I) respectively. Scale bar shown in (H) also applies to (J).

(K–L) Coronal sections of E19.5 mouse brains of the indicated genotypes showing α-pJNK staining by immunofluorescence. Scale bar shown in (K) also applies to (L). Arrows in (H, J, and K-L) point to α-pJNK staining in axonal tracts. (M) Western blots performed with cerebral cortex lysates from animals of the indicated genotypes probed with α-pJNK, α-JNK, and α-GAPDH primary antibodies. (N–O) Bar graph showing the relative pJNK band intensities normalized to the GAPDH band intensities in the indicated genotypes (N) and the relative pJNK/JNK band intensities in the indicated genotypes (O). The values shown are relative to the appropriate controls.

“*” represents statistical significance. Abbreviations: n.s., not significant; CC, corpus callosum.