Figure 7. Simultaneous administration of ALK inhibitor and high-protein diet enhances synaptic growth and adult viability following lysosomal dysfunction.

(A) Bar graph showing the average bouton numbers in animals of the indicated genotypes reared on instant food with the indicated additions.

(B) Mechanism of ALK-dependent block of amino acid uptake in neuronal cells. (C–E) Bars graph showing the average bouton numbers in animals of the indicated genotypes reared on instant food with the indicated additions.

(F) Bar graph showing the percentage of the trpml¹ adults that eclose from their pupal cases when reared on instant food with the indicated additions. The “+” and “−” in (C–F) represent presence or absence of CH5424802 or yeast. Although rearing larvae on instant food resulted in an overall increase in the number of boutons in all the genotypes, the trpml¹, ragC^Δ, and ok>cln3^IR larvae showed a relative decrease in NMJ boutons, and all comparisons were made between animals reared under identical conditions.

(G) Roles of lysosomal function, MTORC1 activity, Wnd/JNK signaling in neurodevelopment.

“*” and “#” represent statistical significance. Abbreviations: n.s., not significant; CH, CH5424802.