Table 1. Genes investigated using quantitative reverse transcription PCR (qRT-PCR).
No. | Gene name and abbreviation | Assay ID | Accession number |
---|---|---|---|
1 | Nuclear factor kappa B (NF-κB) p65 | Mm00501346_m1 | NM_009045.4 |
2 | Inducible nitric oxide synthase (iNOS) | Mm 00440502_m1 | NM_010927.3 |
3 | Tumour necrosis factor alpha (TNF-α) | Mm 00443258_m1 | NM_013693.2 |
4 | Interleukin 10 (IL-10) | Mm 00439614_m1 | NM_010548.2 |
General abbreviation of genes selected for this study and corresponding assay ID and accession number were obtained from the Applied Biosystems website and NCBI database. Assay ID refers to the Applied Biosystems Gene Expression Assays inventoried kits with proprietary primer and TaqMan® probe mix. Assay ID with “Mm” is referred to as “Mus musculus”. All Gene Expression Assay kits indicated are FAM/MGB probed.