1 |
BD GenomeWalker adaptor |
5' GTAATACGACTCACTATAGGGCACGCGTGGTCGACGGCCCG GGCTGGT 3' |
No.1~5 used for the amplification of 5′ flanking sequence of AhLEC1B genome DNA |
2 |
LEC1BGSP1-2 |
5' CCTTGTTCCCATGTAAAACCATGAAAGCA 3' |
3 |
LEC1BGSP2-2 |
5' AGGTAAAGCAGCCGCTAATCTAGTTAGT 3' |
4 |
AP1 |
5' GTAATACGACTCACTATAGGGC 3' |
5 |
AP2 |
5' ACTATAGGGCACGCGTGGT 3' |
6 |
GeneRacer RNA Oligo |
5' CGACUGGAGCACGAGGACACUGACAUGGACUGAAGGAG UAGAAA 3' |
No.6~10 used for the localization of the transcriptional start site of AhLEC1B gene |
7 |
TSS LEC1BGSP1-1 |
5' TCTTTTGCGTCGTCGGAGATTTTAGC 3' |
8 |
TSS LEC1BGSP2 |
same as LEC1BGSP2-2 |
9 |
5' GeneRacer Primer |
5' CGACTGGAGCACGAGGACACTGA 3' |
10 |
5' Nested Primer |
5' GGACACTGACATGGACTGAAGGAGTA 3' |
11 |
BF1 |
5′ AAGCTTTCGTGAATAAAGGAACAC 3′ |
No.11~17 used for the deletion analysis of AhLEC1B promoter |
12 |
BF2 |
5′ AAGCTTACTCTATGATATTCCGAAGG 3′ |
13 |
BF3 |
5′ AAGCTTCCTCGGTTGCATCGCCCT 3′ |
14 |
BF4 |
5′ AAGCTTGCATTGCTTGCAGCTCTTTG 3′ |
15 |
BF5 |
5′ AAGCTTGTTACTCCGTTTCTTCATAC 3′ |
16 |
BR1 |
5′ CCATGGGTAAAGCAGCCGCCAATCTA 3′ |
17 |
BR2 |
5′ CCATGGCTCGCCCTTCGGAATATCAT 3′ |