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. 2015 Oct 1;10(10):e0139213. doi: 10.1371/journal.pone.0139213

Table 1. The primers used in this study.

Serial No. Primer name Sequences Purposes
1 BD GenomeWalker adaptor 5' GTAATACGACTCACTATAGGGCACGCGTGGTCGACGGCCCG GGCTGGT 3' No.1~5 used for the amplification of 5′ flanking sequence of AhLEC1B genome DNA
2 LEC1BGSP1-2 5' CCTTGTTCCCATGTAAAACCATGAAAGCA 3'
3 LEC1BGSP2-2 5' AGGTAAAGCAGCCGCTAATCTAGTTAGT 3'
4 AP1 5' GTAATACGACTCACTATAGGGC 3'
5 AP2 5' ACTATAGGGCACGCGTGGT 3'
6 GeneRacer RNA Oligo 5' CGACUGGAGCACGAGGACACUGACAUGGACUGAAGGAG UAGAAA 3' No.6~10 used for the localization of the transcriptional start site of AhLEC1B gene
7 TSS LEC1BGSP1-1 5' TCTTTTGCGTCGTCGGAGATTTTAGC 3'
8 TSS LEC1BGSP2 same as LEC1BGSP2-2
9 5' GeneRacer Primer 5' CGACTGGAGCACGAGGACACTGA 3'
10 5' Nested Primer 5' GGACACTGACATGGACTGAAGGAGTA 3'
11 BF1 5′ AAGCTTTCGTGAATAAAGGAACAC 3′ No.11~17 used for the deletion analysis of AhLEC1B promoter
12 BF2 5′ AAGCTTACTCTATGATATTCCGAAGG 3′
13 BF3 5′ AAGCTTCCTCGGTTGCATCGCCCT 3′
14 BF4 5′ AAGCTTGCATTGCTTGCAGCTCTTTG 3′
15 BF5 5′ AAGCTTGTTACTCCGTTTCTTCATAC 3′
16 BR1 5′ CCATGGGTAAAGCAGCCGCCAATCTA 3′
17 BR2 5′ CCATGGCTCGCCCTTCGGAATATCAT 3′