Table 1. List of genes used in QRT PCR for the validation of transcriptome data, amplification efficiency and their primer sequence.
| Gene | Amplification efficiency | Forward | Reverse |
|---|---|---|---|
| Glyma16g08340 | 2.07 | ATGTATTCTCAAGACCCAAATG | GAGTTGAGCTTTGTTCAGCAC |
| Glyma18g52480 | 1.84 | AGTGTGGCAGCGTGATCGA | TGGTAGCAGCCTGGTTCTTG |
| Glyma08g05500 | 1.82 | AGCGAAGAGTACAGGAAAGTG | CCTTCTTCAGGTACCCCTTC |
| Glyma03g37940 | 1.90 | GATTTCCCTCTATCGTCACAC | GTTCGGAGTCGCAGGTTGC |
| Glyma13g36870 | 2.02 | GATTTGGCGTGTTTGAACTTTG | GCTGATAATTGTTGTTGCTACC |
| Glyma13g35560 | 2.05 | TGAGGATGTGGCGGGTACC | TCCATCACCTATTGACCCCAAT |
| Glyma17g17210 | 1.90 | CGATGCCACCAACACCAGTG | TGGAGAAGCTGCTGAGGCTT |
| Glyma0892s00200.1 | 2.00 | TAAGGGACACACCAAGCGAA | CAACGTGTCCATCCTTATCC |
| Glyma05g35050 | 1.80 | CAAAGAATCATCTTCTTCAGC | GTTGAATGCTGAGAAACACC |
| Glyma10g33060 | 2.00 | AACCGAGGAGCACTCCGG | CCGCCGTACGCCGCG |
| XM_003528209.2 | 2.02 | GCC TCT GGA TAC CTG CTC AAG | ACC TCC TCC TCA AAC TCC TCT G |