Fig 2. A miRNA-like long hairpin producing phased 21~22-nt ta-siRNAs.

(A) Abundant small RNAs were generated from ten phases of the long hairpin, with red and blue bars representing sequences from the 5′ and 3′ arms, respectively. Their corresponding abundances are shown above as columns in the same color. (B) Degradome analysis confirmed the cleavage of target mRNAs guided by 3′_D2 and 5′_D8. The t-plots showed absolute sequence abundances over the target mRNAs, with red arrows indicating sequence reads consistent with siRNA-directed cleavage. Representative data origins from degradome libraries I or II are also indicated in the t-plots.