Table 2. Newly identified 37 miRNAs in four small RNA libraries.
miRNA ID | Len | Sequence (5′–3′) | TPM ‡ | miR* ║ | Precursor | |||
---|---|---|---|---|---|---|---|---|
(nt) | 7 DPA | 14 DPA | 21 DPA | 28 DPA | (EST No.) | |||
tae-miR2009b.2 | 22 | UGAGAAGGCAGAUCAUAAUAGC | 67.53 | 57.24 | 32.02 | 73.24 | Y | DR736484 |
tae-miR2009c.2 | 22 | UGAGAAGGCAGAUCAUAAUAGC | 67.53 | 57.24 | 32.02 | 73.24 | Y | CK193889 |
tae-miR5048.2 | 22 | UAUAUUUGCAGGUUUUAGGUCU | 138.74 | 309.97 | 104.25 | 243.10 | N | CJ629474 |
tae-miR5175b | 21 | UUCCAAAUUACUCGUCGUGAU | 3.09 | 13.54 | 1.76 | 1.78 | N | HX015772 |
tae-miR5175c | 21 | UUCCGAUUUACUCGUCGUGGU | 0.35 | 0.20 | 0.20 | 0.00 | N | HX013251 |
tae-miR9654c | 22 | UUCCGAAAGGCUUGAAGCAAAU | 2.45 | 4.4 | 0.03 | 0.4 | N | CA737852 |
tae-miR9655b | 22 | UGGCUACUUCCUUUCCCUUGCC | 72.08 | 65.12 | 49.38 | 49.25 | N | CV779317 |
Ta-miRn1 | 21 | UUUCUUCUUCCUCUUCCUACC | 31.69 | 8.14 | 2.38 | 0.30 | N | CD935885 |
Ta-miRn2 | 21 | UUGAGUCAUCUAUUUUGGAAC | 7.53 | 5.54 | 3.85 | 4.06 | Y | DR739812 |
Ta-miRn3a | 21 | UGGACGAGUAAUUCCGAACGG | 9.28 | 9.81 | 8.88 | 2.97 | N | CD881394 |
Ta-miRn3b | 21 | UAGACGAGUAAUUCCGAACGG | 2.45 | 2.34 | 0.88 | 0.00 | N | BJ249702 |
Ta-miRn4a | 21 | UCUGUAAACUAAUAUAAGAGC | 6.71 | 8.27 | 2.02 | 0.00 | Y | CJ706003 |
Ta-miRn4b | 21 | UCUGUAAACUAAUAUAAGAGC | 6.71 | 8.27 | 2.02 | 0.00 | Y | CJ788080 |
Ta-miRn5 | 21 | UCACAAAUAUAAGAUGUUCUG | 4.96 | 4.47 | 3.69 | 3.07 | N | JV876054 |
Ta-miRn6a | 21 | UCUUCUGUAGAAAUAGGCACC | 7.30 | 43.70 | 36.49 | 14.17 | N | HX200139 |
Ta-miRn6b | 21 | UCUCCUGUAGAAAUAGGCACC | 24.11 | 106.55 | 82.80 | 31.91 | N | CA644455 |
Ta-miRn7a | 21 | UUAGAGAUUUCAAUAUGGACU | 23.58 | 41.10 | 50.49 | 34.69 | N | BJ268299 |
Ta-miRn7b | 21 | UUAGAGAUUUCAAUACGGACU | 7.88 | 15.48 | 21.80 | 19.03 | N | BJ273584 |
Ta-miRn7c | 21 | UUAGAGAUUCCACUACGGACU | 4.55 | 8.87 | 6.89 | 6.05 | N | HX036959 |
Ta-miRn8a | 21 | CUCCGUUCCAAAAUAGAUGAC | 115.74 | 60.71 | 14.75 | 5.15 | Y | HX035580 |
Ta-miRn8b | 21 | CUCCGUUCCAAAAUAGAUGAC | 115.74 | 60.71 | 14.75 | 5.15 | Y | HX163183 |
Ta-miRn8c | 21 | CUCCGUUCCAAAAUAGAUGAC | 115.74 | 60.71 | 14.75 | 5.15 | Y | HX032309 |
Ta-miRn9 | 21 | CAAGUUAUGCAGUUGCUGCCU | 9.51 | 0.47 | 0.00 | 0.00 | N | HP621130 |
Ta-miRn10 | 21 | CCACGACGAGUAAAUCGGAAC | 3.09 | 2.80 | 1.21 | 0.00 | N | CJ849101 |
Ta-miRn11 | 24 | ACGGACUCCCCGCAGCCUCCACCC | 20.08 | 21.82 | 8.68 | 16.95 | N | GH731699 |
Ta-miRn12 | 21 | AUCCAUAUUAGUUGUCGCUGA | 5.19 | 4.80 | 1.37 | 1.49 | N | CA632670 |
Ta-miRn13a | 21 | UUUAGAGAUUUCAAAUGGACU | 17.74 | 55.37 | 207.45 | 183.34 | Y | CD887655 |
Ta-miRn13b | 21 | UUUAGAGAUUUCAAAUGGACU | 17.74 | 55.37 | 207.45 | 183.34 | Y | CV782135 |
Ta-miRn14 | 21 | UUCGGAAUUACUUGUCGCAGA | 8.46 | 7.81 | 12.66 | 15.16 | Y | CJ610100 |
Ta-miRn15 | 21 | AUUACUUGUCUUGGAUUUGUC | 20.02 | 10.87 | 19.81 | 0.10 | Y | CJ786514 |
Ta-miRn16 | 21 | AUUACUCGUCGCAGAAAUGGA | 8.58 | 3.74 | 8.09 | 8.32 | N | CD907788 |
Ta-miRn17 | 21 | CAUUUCCGAGACAAGUAAUUC | 52.35 | 27.89 | 8.52 | 0.00 | Y | CK193033 |
Ta-miRn18 | 21 | UCCGUCCCAUAAUAUAAGAGC | 4.26 | 2.74 | 0.72 | 1.09 | Y | CJ631979 |
Ta-miRn19a | 20 | UUUAUCACCGCCUCUUUCUC | 21.83 | 8.61 | 3.26 | 0.40 | Y | GAEF01081951 |
Ta-miRn19b | 20 | UUUAUCACCGCCUCUUUCUC | 21.83 | 8.61 | 3.26 | 0.40 | Y | GAEF01081952 |
Ta-miRn20 | 21 | UCGAGAUCCAACGGCUGAGGU | 8.29 | 6.14 | 2.58 | 5.85 | N | JW008285 |
Ta-miRn21 | 21 | UGUCUCGGAUAUGGAUGUAUC | 2.86 | 2.94 | 9.69 | 20.91 | Y | JV914483 |
‡ TPM: transcripts per million. miRNA abundance was scored according to the reads of defined miRNAs and their ± 2 nt variants on the precursors.
║ Y: miRNA* species (or ± 1 nt variants) for their corresponding miRNAs were sequenced in our small RNA libraries. N: miRNA* unsequenced.