Table 1.
Forward and reverse primer sequences for quantitative real-time PCR
| Primer Sequence (5′ → 3′) |
|||
|---|---|---|---|
| Gene Description | Protein Name | Forward | Reverse |
| 18S Ribosomal RNA | 18S | TCAAGAACGAAAGTCGGAGG | GGACATCTAAGGGCATCAC |
| Ribosomal protein S13 | RSP13 | TGCCGTTTCCTACCTCGTTC | CACGTCGTCAGACGTCAACT |
| Tumor necrosis factor-α | TNF-α | AGCCGATGGGTTGTACCTTG | ATAGCAAATCGGCTGACGGT |
| Interleukin 6 | IL-6 | TCCAGTTGCCTTCTTGGGAC | AGTCTCCTCTCCGGACTTGT |
| Monocyte chemoattractant protein 1 | MCP1 | GGCTGGAGAGCTACAAGAGG | GGTCAGCACAGACCTCTCTC |
| Interferon-γ | IFN-γ | AGCAAGGCGAAAAAGGATGC | TCATTGAATGCTTGGCGCTG |
| Chemokine (C–C motif) receptor 2 | CCR2 | ACACTCCTGGCTGGAAAAGAAG | GGCATTTACAGGTATGGCTCCT |
| Chemokine (C–C motif) ligand 5 | RANTES | TCCCTGTCATTGCTTGCTCT | ATTTTCCCAGGACCGAGTGG |
| Leptin | Leptin | CCTATTGATGGGTCTGCCCG | TGAGCGCTACCTGCATAGAC |
| Adiponectin | Adipoq | GCACTGGCAAGTTCTACTGCAA | GTAGGTGAAGAGAACGGCCTTGT |
| CD68 antigen | CD68 | TGTTCAGCTCCAAGCCCAAA | GTACCGTCACAACCTCCCTG |
| Integrin α X | CD11c | ATGCCACTGTCTGCCTTCAT | GTCTCCTTGAAGATGGCCCG |
| EGF-like module-containing mucin-like hormone receptor-like 1 | F4/80 | GGGTATCATGAGTTGATGGCAGT | CCTTGGTGCATGAAACTCCT |
| Integrin α M | CD11b/CD18 | AGCTTGGCTTTTTCAAGCGG | AAAGGCCGTTACTGAGGTGG |
| Cytochrome b-245, α polypeptide | p22phox | ACTCTATCGCTGCAGGTGTG | AAGCTTCACCACAGAGGTCAG |
| Neutrophil cytosolic factor 1 | p47phox | GGCACAAAGGACAATCCATCG | TTCCGTTTGGTGCTCTCTGT |
| DNA damage-inducible transcript 3 | CHOP | ATGTTGAAGATGAGCGGGTG | TGGAACACTCTCTCCTCAGGT |
| Heat shock protein 5 | GRP78 | TGTGTGTGAGACCAGAACCG | GCAGTCAGGCAGGAGTCTTA |