Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 2;9:383. doi: 10.3389/fncel.2015.00383

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Bifari, Berton, Pino, Kusalo, Malpeli, Di Chio, Bersan, Amato, Scarpa, Krampera, Fumagalli and Decimo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Nestin⁺ and Ki67⁺ cells are present in leptomeninges. (A) Immunostaining of nestin (red, left column) and nestin (red)/Ki67 (green, right column) at different stages of development; from top to bottom: E20, P0, P15, adult. Confocal microscopy analysis revealed that nestin⁺ cells (red) are present in the leptomeninges from embryonic stage E20 up to adulthood. Nuclei are stained with TO-PRO3 (blue). Scale bar: 50 μm. (B,C) Immunoperoxidase staining (brown) with anti-nestin antibody of brain sections. (B) Coronal section; the white dashed line highlights the border between neural parenchyma and meninges. (C) En face view showing nestin⁺ cells as an intricate net covering the brain. (D) Quantification of nestin⁺ cells normalized for the total number of nuclei in meninges. (E) Percentage of nestin⁺/Ki67⁺ cells; the values are normalized for the number of nestin⁺ cells. In (D,E), values are mean ± SD.