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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Nat Cell Biol. 2015 Aug 24;17(10):1317–1326. doi: 10.1038/ncb3233

Figure 6. Pyruvate carboxylase supports growth of Sdhb-null cells by sustaining aspartate biosynthesis.

Figure 6

(a) Citrate abundance in PCX-silenced cells cultured for 24h with U-13C-glucose. Data are presented as mean±s.e.m of n=9 wells pooled from three independent experiments. (b) Effect of PCX silencing on the total intracellular palmitate levels. Data are presented as mean±s.e.m (n=3 wells) of one experiment, performed once. (c) Number of PCX-silenced cells in the presence (+) or absence (−) of 2.5 mM aspartate measured after 96h of culture. Data are presented as mean±s.e.m (n=4 wells) of one experiment, performed once. (d) Number of control and PCX-silenced cells supplemented with 50 μM palmitate measured after 96h of culture. Data are presented as mean±s.e.m (n=4 wells) of one experiment, performed once. Raw data of independently repeated experiments are provided in Supplementary Table 3.