Extended Data Table 1.
Refinement and model statistics.
| UAA | UAG | UGA | |
|---|---|---|---|
| Data Collection | |||
| Particles | 49,979 | 20,515 | 22,058 |
| Pixel size (Å) | 1.34 | 1.34 | 1.34 |
| Defocus range (μm) | 1.1-5.9 | 0.7-4.1 | 0.7-3.8 |
| Defocus mean (μm) | 3.2 | 2.4 | 2.3 |
| Voltage (kV) | 300 | 300 | 300 |
| Electron dose (e- Å−2) | 30 | 30 | 30 |
| Model composition | |||
| Non-hydrogen atoms | 226,532 | 226,533 | 226,533 |
| Protein residues | 12,676 | 12,676 | 12,676 |
| RNA bases | 5,820 | 5,820 | 5,820 |
| Ligands (Zn2+/Mg2+/ADP) | 8/197/2 | 8/197/2 | 8/197/2 |
| Refinement | |||
| Resolution (Å) | 3.65 | 3.45 | 3.83 |
| Map sharpening B-factor (Å2) | −81.7 | −50.6 | −82.7 |
| Average B factor (Å2) | 105.8 | 87.4 | 93.5 |
| FSCaverage | 0.85 | 0.84 | 0.88 |
| FSCaverage (eRF1) | 0.70 | 0.64 | 0.74 |
| FSCaverage (ABCE1) | 0.71 | 0.62 | 0.75 |
| R.m.s. deviations | |||
| Bond lengths (Å) | 0.006 | 0.006 | 0.008 |
| Bond angles (°) | 1.19 | 1.22 | 1.40 |
| Validation | |||
| Molprobity score | 2.7 (93rd percentile) | 2.8 (89th percentile) | 3.0 (89th percentile) |
| Clashscore, all atoms | 5.2 (100th percentile) | 6.2 (97th percentile) | 8.2 (97th percentile) |
| Good rotamers (%) | 88.2 | 87.5 | 86.4 |
| Ramachandran plot | |||
| Favored (%) | 87.0 | 85.8 | 83.5 |
| Outliers (%) | 3.3 | 3.5 | 4.2 |
| Validation (RNA) | |||
| Correct sugar puckers (%) | 96.5 | 93.4 | 96.0 |
| Good backbone conformations (%) | 68.2 | 66.8 | 65.8 |