Table 1.
B. subtilis strains used in this study.
| Strain | Genotype | Source or reference |
|---|---|---|
| E. coli MC1061 | F−araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 hsdR2 () mcrA mcrB1 rpsL (Strr) | Laboratory stock, (Casadaban and Cohen, 1980) |
| B. SUBTILIS | ||
| 168 | trpC2 | Bacillus Genetic Stock Center, (Zeigler et al., 2008) |
| DOW41 | trpC2 ccpA::specr | This study |
| DOW42 | DOW41 with pHT304 | This study |
| DOW43 | DOW41 with pWH2422-ccpA-wt | This study |
| DOW44 | DOW41 with pWH2422-ccpA-M17R | This study |
| DOW45 | DOW41 with pWH2422-ccpA-T62H | This study |
| DOW46 | DOW41 with pWH2422-ccpA-R304W | This study |