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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Arthritis Rheumatol. 2015 May;67(5):1214–1220. doi: 10.1002/art.39027

Figure 2.

Figure 2

Comparison of cell surface receptor and lymphocyte subset percentages in uninfected, CHIKV-infected, and untreated RA patients from two experiments. (A) Cell counts for each subset were derived from sequential manual gating of CyTOF data based on multiple cell surface marker expression. Representative data points from individual patients are shown with the mean percentage depicted by a line. One patient with CHIKV arthritis was unable to donate sufficient blood for PBMC collection. The results were validated by two independent CyTOF experiments performed using separate PBMC aliquots from patients with CHIKV arthritis (n=7), untreated RA (n=6; four seropositive and two seronegative RA), and healthy control subjects (n=4). Statistical significance was tested by one-way ANOVA followed by Bonferroni's post-test (*, p<0.05). (B) Representative SPADE plots from 3 different patient groups (left panel, control; middle panel, CHIKV; right panel, RA) showing expression of L-selectin (CD62L) are presented. Clustering of cell groups was performed manually. Relative fold-expression is indicated by the color scale. Each circle represents a cluster of cells expressing similar levels of all 22 markers and its relation to other cluster of cells expressing similar combination of 22 markers. The size of the circle correlates with the number of cells in each cluster.